Question

Raju is a diabetic parient who takes insulin injections regulary. The insulin used by such patients is producted by genetically engineered organisms . Write the different steps involved in the production of insulin by genetic engineering.

Answer 1

(1) Preparation of DNA sequences corresponding to A&B chain of human insulin.

(2) Introduce them in plasmid of E.coli. 

(3) Products of A&B Chains are separated. 

(4) Combine A&B chains by creating disulphide bonds