The changes in DNA during reproduction are mainly responsible for evolution. The changes which take place in the DNA of species go on accumulating from one generation to the next. So, if the changes in the DNA of any two species are less, then the two species are quite close to one another in evolutionary terms. But if the changes in the DNA of two species are much more, then the two species will be far apart from one another in evolutionary terms. Thus, it is the characteristic of the extent of change in the DNA which is being used to determine how close two species are in evolutionary terms.