1. Agrobacterium tumefaciens is a soil bacterium that causes crown gall disease in plants.
2. This disease involves the formation of tumor in the plant.
3. Ti plasmid in A. tumefaciens contains a transposon called T DNA.
4. T DNA inserts copies of itself into the chromosomes of infected plant cells.
5. The transposons, with the foreign DNA, can be inserted into the host cell’s chromosomes.
6. A plant cell containing this DNA, can then be grown in culture or induced to form a new, transgenic plant.
construction of Genomic library:
1. When genomic library is constructed, the entire genome or DNA is isolated from a particular organism.
2. This DNA is fragmented using suitable restriction endonucleases.
3. These separated fragments are later inserted into cloning vectors.
4. This develops recombinant vectors.
5. Such recombinant vectors are transferred into suitable organisms such as bacteria or yeast. Each host cell then contains one fragment.
6. These transformed organisms are cultured and their clones are thus produced. These clones are stored in the genomic library.
