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What is the cause of release of unusually large energies in nuclear fission reactions? How is the energy per fission calculated?

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During the nuclear fission process a heavy nucleus splits to form particles. As we know that the subatomic particles are held together by strong nuclear forces and when the particle breaks it releases a tremendous amount of energy. The energy released during the fission reaction is calculated by using the famous relation: 

E = mc2 

Where:

E = Energy released 

m = mass defect = Total Mass before the reaction – Total mass after the reaction 

c = speed of light

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